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Image Search Results
Journal: Communications Biology
Article Title: Lysine malonylation regulates human sperm motility
doi: 10.1038/s42003-026-09683-y
Figure Lengend Snippet: A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: PRM2 for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.
Article Snippet: Anti-ACTIN antibody (66009-1-Ig), anti-SIRT5 antibody (67257-1-Ig), anti-acetyl-CoA carboxylase 1 (ACC1) antibody (21923-1-AP), anti-fatty acid synthase (FASN) antibody (10624-2-AP),
Techniques: Isolation, Western Blot, Staining, Immunofluorescence, Confocal Microscopy, Microscopy
Journal: Communications Biology
Article Title: Lysine malonylation regulates human sperm motility
doi: 10.1038/s42003-026-09683-y
Figure Lengend Snippet: The levels of Kmal and SIRT5 were analyzed in 53 normozoospermic men (NOR) and 41 asthenozoospermic men (AST) by western blot. A An example illustrating the presence of sperm Kmal and SIRT5 in AST and NOR. B Semi-quantitative analysis of Kmal in NOR and AST was conducted as outlined in the “Materials and methods” section. The red box indicates 10 AST whose sperm Kmal levels exceeded 1.51, the highest value of relative Kmal levels in NOR. C Correlations between sperm Kmal and progressive motility in 94 samples were evaluated through linear regression analysis. The glycolysis ( D ) and ATP level ( E ) were examined in 10 AST indicated in the red box in Fig. 3B. F Semi-quantitative analysis of SIRT5 in NOR and AST was conducted as outlined in the “Materials and methods” section. G Correlations between sperm Kmal and SIRT5 in 94 samples were assessed through linear regression analysis. The error bars represent the means ± SEM. The differences between NOR and AST were analyzed by unpaired t-test with statistical significance indicated by * p < 0.05 and *** p < 0.001.
Article Snippet: Anti-ACTIN antibody (66009-1-Ig),
Techniques: Western Blot